Preliminary Phytochemical Screening, Chemical Investigation, Enzyme Inhibiting Activity and Atomic Absorption Spectrophotometric Determination of Minerals of Plant Extracts of Scoparia Dulcis.linn

The phytochemical studies on the plant of Scoparia dulcis.Linn resulted in isolation of α and β amyrin and steroidal glycoside are being reported for the first time from this plants. The preliminary phyto chemical screening showed the presence of Carbohydrates, protein and aminoacids, glycosides, phenolic compounds, steroids, di and tri terpenes, and flavanoids. The plant is reported to contain flavonoids, which was evident from the phytochemical investigation of crude extract, an attempt was made to identify these flavonoids by paper chromatography. It is stated that phenols readily complex with protein and as a result there is often inhibition of enzyme activity. Since this plant contains phenolic derivatives an attempt was made for the possible enzyme inhibitory action. The alcohol and aqueous extract exhibited high degree of inhibition towards the enzymes tested. These compounds have been characterized on the basis of spectral and other data. The isolated compounds (BJ I, BJ II, BJ III, BJ IV and BJ V ) from column chromatography. An attempt to estimate the amount of magnesium present in the plant by atomic absorption spectrophotometry, was made and the presence of an appreciable amount of magnesium was established. The ash content of the sample was 7% and the magnesium concentration was found to be 41.342mg/L. that is the percentage of magnesium content in the air dried crude drug is 0.567%.